Journal: BMC Veterinary Research

Article Title: Long-term changes of Th17 and regulatory T cells in peripheral blood of dogs with spinal cord injury after intervertebral disc herniation

doi: 10.1186/s12917-023-03647-8

Figure Lengend Snippet: Illustration of a Treg and a Th17 cell analysis using flow cytometry Results of Treg and Th17 cell analysis of a dog after recovery of spinal cord injury using MACSQuant analysis software In the prior performed isotype controls, limits were set at approx. 2% of the cells [ , – ]. This limit was transferred to the test fractions (pink lines) in each case to detect the percentages of FOXP3 (a) or IL17 (b) positive cells, which are located to the right of the pink line Absolute cell numbers were subsequently calculated to make the analyses comparable a) The graphic displays the percentage of FOXP3 positive cells for detection of Treg cells. 9.56% FOXP3 positive cells were identified b) The graphic displays the percentage of IL17 positive cells for detection of Th17. 16.20% IL17 positive cells were identified. The results of a stimulated Th17 cell fraction are shown

Article Snippet: For cell fixation, a flow cytometry fixation buffer (R&D Systems®, Minneapolis, Minnesota, USA) was used.

Techniques: Cell Analysis, Flow Cytometry, Software

Journal: International archives of allergy and immunology

Article Title: Importance of NK Cells in Cellular and Humoral Responses Triggered by Pneumococcus Vaccination.

doi: 10.1159/000535562

Figure Lengend Snippet: Fig. 5. Comparison of cytokine secretion levels. Splenocytes obtained 4 weeks after the last dose of combined pneumococcal vaccination were stimulated with PPV-23 vaccine antigen in vitro, and secretion levels of IFN-γ (a), IL-4 (b), and IL-17 (c) cytokines were quantified by ELISA assay of the supernatants collected. None of the cytokines displayed statistically significant difference in secretion levels between control (NK+) and NK cell- depleted (NK−) mice.

Article Snippet: This is followed by fixation and permeabilization of splenocytes with Fixation Buffer (Cytek Biosciences) and Perm Buffer (Cytek Biosciences), respectively.

Techniques: Comparison, In Vitro, Enzyme-linked Immunosorbent Assay, Control

Journal: International archives of allergy and immunology

Article Title: Importance of NK Cells in Cellular and Humoral Responses Triggered by Pneumococcus Vaccination.

doi: 10.1159/000535562

Figure Lengend Snippet: Fig. 4. Comparison of CD4+ T-cell subset levels. Splenocytes collected 4 weeks after the PPV-23 vaccination were stimulated by PPV-23 vaccine antigen for 24 h. Antibodies against IFN-γ, IL-4, and IL-17 were utilized to detect the frequencies of TH1 (a), TH2 (b), and TH17 (c) cells among CD4+ T-cell population, respectively, by flow cytometry analysis. While TH1 percentages were reduced, TH2 and TH17 cell ratios stayed unaltered in NK cell- depleted (NK−) mice in comparison to the control mice (NK+). ** represents p < 0.01.

Article Snippet: This is followed by fixation and permeabilization of splenocytes with Fixation Buffer (Cytek Biosciences) and Perm Buffer (Cytek Biosciences), respectively.

Techniques: Comparison, Cytometry, Control